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Teilnahme an der Veranstaltung per Zoom (Meeting ID: 680 8031 9896, Passcode: DPhG) oder in Präsenz im großen Hörsaal der Pharmazeutischen Institute, Corrensstraße 48 in Münster
Multidrug-resistant gram-negative pathogens like Escherichia coli have become increasingly challenging to treat, necessitating the exploration of alternative treatment options. Targeting virulence factors such as biofilm formation could be one such avenue. Inhibition of biofilm-related structures like curli and cellulose formation in E. coli has been demonstrated with various phenolicnatural compounds, such as epigallocatechin gallate. To assess whether structurally distinct compounds, namely octyl gallate, scutellarein, and wedelolactone influence common pathways of biofilm formation in E. coli, a comprehensive comparative RNA-sequencing approach was adopted, supplemented by RT-qPCR to gain initial insights into the affected pathways at the transcriptomic level. Bioinformatic analysis of the RNA-Seq data was conducted using DESeq2, BioCyc, and KEGGMapper. The comparative bioinformatics analysis of pathways revealed that, regardless of their structure, all compounds predominantly impacted similar biological processes. These pathways included bacterial motility, chemotaxis, biofilm formation, as well as metabolic processes such as arginine biosynthesis and the tricarboxylic acid cycle. Overall, this study provides the initial insights into the potential mechanisms of action of novel phenolic biofilm inhibitors and underscores the intricate regulatory processes involved in biofilm formation in E. coli.
